HORTICULTURE

Detailed explanation-1: -The third instar, which is the ultimate stage of larval development, and has prolonged duration, is the stage most often used for identification, if the appropriate taxonomic key is available. The adult fly, reared from the third instar, also is often used to confirm identity.

Detailed explanation-2: -Drosophila third-instar larvae exhibit changes in their behavioral responses to gravity and food as they transition from feeding to wandering stages. Using a thermal gradient encompassing the comfortable range (18°C to 28°C), we found that third-instar larvae exhibit a dramatic shift in thermal preference.

Detailed explanation-3: -A fly life cycle is very similar to that of most insects. It proceeds as a basic cycle that begins with an egg, followed by development through a larva phase, a pupa phase, and finally, turning into an adult.

Detailed explanation-4: -Midway through the third and final instar, larvae stop feeding and enter “wandering stage” when they exit the food source completely in search of an appropriate pupation site.