HORTICULTURE

Detailed explanation-1: -The core principle of biotechnology involves harnessing biological systems and organisms, such as bacteria, yeast, and plants, to perform specific tasks or produce valuable substances. Key techniques include genetic engineering, tissue culture, and fermentation.

Detailed explanation-2: -Biotechnology is any technique that uses living organisms or parts of organisms to make/modify products, improve plants or animals, or develop microorganisms for specific use [14].

Detailed explanation-3: -DNA sequencing is the gold standard for microorganism identification. The 16S ribosomal RNA (rRNA) gene is the most common sequencing target for bacteria and is 1542 base pairs (bp) in length.

Detailed explanation-4: -The principal way a microbiologist studies microorganisms is by observing them through a microscope.

Detailed explanation-5: -The Crowded Plate Technique is the simplest screening technique employed in detecting and isolating antibiotic producers. It consists of preparing a series of dilutions of the source material of the antibiotic producing micro organisms, followed by spreading the dilution on the agar plates.