HORTICULTURE SCIENCE
PLANT BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The bacteria have been grown on a petri dish containing insulin.
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The bacteria have been genetically engineered to contain the DNA needed to produce insulin.
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The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.
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The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.
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Detailed explanation-1: -This process is called recombinant technology. The bacterial DNA now contains the human insulin gene and is inserted into a bacteria. When the bacterial cells reproduce, the human insulin gene is also reproduced in the cells and produces protein by transcription and translation process.
Detailed explanation-2: -Recombinant human insulin has been produced predominantly using E. coli and Saccharomyces cerevisiae for therapeutic use in human.
Detailed explanation-3: -Eli Lilly prepared the two DNA sequences complementary to the A chain and B chain of insulin. These DNA sequences were incorporated in the plasmids of E. coli. These two chains were synthesizes separately, extracted and combined by disulphide bonds to form genetically engineered human insulin.