HORTICULTURE SCIENCE
PLANT BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Construction recombinant DNA by joining with cloning vectors
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Cutting of DNA into fragments
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separation of DNA fragments according to their size
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Isolation of DNA molecules
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Detailed explanation-1: -Traditional agarose gels are most effective at the separation of DNA fragments between 100 bp and 25 kb. To separate DNA fragments larger than 25 kb, one will need to use pulse field gel electrophoresis6, which involves the application of alternating current from two different directions.
Detailed explanation-2: -Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.
Detailed explanation-3: -Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.
Detailed explanation-4: -Agarose gel analysis is the most commonly used method for analyzing DNA fragments between 0.1 and 25 kb, while pulse-field gel electrophoresis enables analysis of DNA fragments up to 10, 000 kb.