HORTICULTURE

HORTICULTURE SCIENCE

PLANT BIOTECHNOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Gel electrophoresis separates DNA based on its
A
shape
B
diameter
C
length
D
numbers of A’s
Explanation: 

Detailed explanation-1: -Because DNA has a uniform mass/charge ratio, DNA molecules are separated by size within an agarose gel in a pattern such that the distance traveled is inversely proportional to the log of its molecular weight(3).

Detailed explanation-2: -Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

Detailed explanation-3: -Electrophoretic mobilities of DNA molecules ranging in length from 100 to 10 000 base pairs (bp) were measured in gels of eleven concentrations of agarose from 0.5 to 1.5%.

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