HORTICULTURE

Detailed explanation-1: -Restriction enzymes can be isolated from bacterial cells and used in the laboratory to manipulate fragments of DNA, such as those that contain genes; for this reason they are indispensible tools of recombinant DNA technology (genetic engineering).

Detailed explanation-2: -Restriction enzymes are isolated from the bacteria that manufactures them also. They protect the bacteria from the attacking virus (bacteriophage).

Detailed explanation-3: -Type I restriction enzymes were the first to be identified and were first identified in two different strains (K-12 and B) of E. coli. These enzymes cut at a site that differs, and is a random distance (at least 1000 bp) away, from their recognition site.