HORTICULTURE SCIENCE
PLANT BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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3%
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30%
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60%
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90%
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Detailed explanation-1: -The Process of DNA Microinjection In DNA microinjection, also known as pronuclear microinjection, a very fine glass pipette is used to manually inject DNA from one organism into the eggs of another. The best time for injection is early after fertilization when the ova have two pronuclei.
Detailed explanation-2: -Even with the direct nuclear approach, microinjection is a laborious procedure. Only one cell can be injected at a time, and many injections are required before getting a successful transgene expression.
Detailed explanation-3: -Gordon and Dr. Ruddle also demonstrated the pronuclear microinjection technique and their application to develop stable transgenics by insertion of recombinant DNA into the embryonic genome (Gordon et al. 1980).