HORTICULTURE

HORTICULTURE SCIENCE

PLANT BIOTECHNOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which chemicals are used to cut DNA into fragments for a gel electrophoresis procedure?
A
enzymes
B
hormones
C
molecular bases
D
ATP molecules
Explanation: 

Detailed explanation-1: -In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences.

Detailed explanation-2: -Restriction Enzyme A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end.

Detailed explanation-3: -Hazardous chemicals commonly used in conjunction with electrophoresis work include: Ethidium bromide – mutagen, irritant. Acrylamide – carcinogen, neurotoxin, irritant. Phenol – corrosive, toxic.

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