HORTICULTURE

HORTICULTURE SCIENCE

PLANT BIOTECHNOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following best describes “gel electrophoresis?"
A
A tool for separating chunks of DNA
B
A tool for editing someone’s genes
C
A tool for adding genes from a new species into an organism’s DNA
D
Copying DNA to make an identical organism
Explanation: 

Detailed explanation-1: -Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.

Detailed explanation-2: -DESCRIPTION. Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight. Smaller fragments migrate faster than larger ones; the distance migrated on the gel varies inversely with the logarithm of the molecular weight.

Detailed explanation-3: -Gel electrophoresis is most commonly used for separation and purification of proteins and nucleic acids that differ in size, charge, or conformation. The gel is composed of polyacrylamide or agarose. Agarose is appropriate for separating DNA fragments ranging in size from a few hundred base pairs to about 20 kb.

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