HORTICULTURE SCIENCE
PLANT BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Recombinant DNA is transferred to a bacterial cell
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A specific gene in a DNA sequence is identified.
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The DNA segment is combined into a plasmid.
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The DNA segment to be inserted is isolated.
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Detailed explanation-1: -Which procedure is the final step in the production of a transgenic organism? Recombinant DNA is transferred to a bacterial cell.
Detailed explanation-2: -Insertion of recombinant DNA into the host The host is the final tool of rDNA technology, which consumes the vector engineered with the desired DNA with the aid of the enzymes. Insertion of the desired recombinant DNA into the host organism can be achieved in various ways.
Detailed explanation-3: -Transfer of this DNA to another cell is referred to as transduction. Transferred DNA once inside the infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into the host bacterium’s genome through homologous or site directed recombination.
Detailed explanation-4: -Isolation of gene and vector (by PCR) Digestion of gene and vector (by restriction endonuclease) Ligation of gene and vector (by DNA ligase) Selection and expression of transgenic construct.