HORTICULTURE

Detailed explanation-1: -Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used, to produce clones of a plant in a method known as micropropagation.

Detailed explanation-2: -Plant tissue culture in a controlled environment drastically reduces pathogens, fungi, and other microbes that interfere with reproductive mechanisms. Sterile conditions require the complete absence of microorganisms including bacteria, fungi, yeast, and their spores.

Detailed explanation-3: -Sterile conditions are maintained to prevent contamination with microorganisms. Cultures are sometimes started from single cells, resulting in the production of uniform biological populations called clones. Single cells typically give rise to colonies within 10 to 14 days of being placed under culture conditions.