MUSCLE PHYSIOLOGY

Detailed explanation-1: -The myosin head then rebinds at a new position on the actin filament, resulting in the release of ADP and Pi and triggering the “power stroke, ‘’ in which the myosin head returns to its initial conformation, thereby sliding the actin filament toward the M line of the sarcomere .

Detailed explanation-2: -At the end of the power stroke, the myosin is in a low-energy position. After the power stroke, ADP is released, but the cross-bridge formed is still in place. ATP then binds to myosin, moving the myosin to its high-energy state, releasing the myosin head from the actin active site.

Detailed explanation-3: -ATP first binds to myosin, moving it to a high-energy state. The ATP is hydrolyzed into ADP and inorganic phosphate (Pi) by the enzyme ATPase. The energy released during ATP hydrolysis changes the angle of the myosin head into a “cocked” position, ready to bind to actin if the sites are available.

Detailed explanation-4: -The myosin head moves toward the M line, pulling the actin along with it. As the actin is pulled, the filaments move approximately 10 nm toward the M line. This movement is called the power stroke, as it is the step at which force is produced.

Detailed explanation-5: -Step 3: ATP binding also causes a large conformational shift in the ‘lever arm’ of myosin that bends the myosin head into a position further along the filament. ATP is then hydrolysed, leaving the inorganic phosphate and ADP bound to myosin.