MUSCLE PHYSIOLOGY

Detailed explanation-1: -PFK1 is the most important control site in the mammalian glycolytic pathway. This step is subject to extensive regulation since it is not only highly exergonic under physiological conditions, but also because it is a committed step-the first irreversible reaction unique to the glycolytic pathway.

Detailed explanation-2: -Because phosphofructokinase (PFK) catalyzes the ATP-dependent phosphorylation to convert fructose-6-phosphate into fructose 1, 6-bisphosphate and ADP, it is one of the key regulatory steps of glycolysis.

Detailed explanation-3: -Regulation of glycolysis Several steps in glycolysis are regulated, but the most important control point is the third step of the pathway, which is catalyzed by an enzyme called phosphofructokinase (PFK).

Detailed explanation-4: -phosphofructokinase, enzyme that is important in regulating the process of fermentation, by which one molecule of the simple sugar glucose is broken down to two molecules of pyruvic acid.

Detailed explanation-5: -Phosphofructokinase-1 (PFK-1) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from ATP to fructose-6-phosphate (F6P) to yield ADP and fructose-1, 6-bisphosphate (FBP). See Glycolysis Enzymes. Mg2+ is also important in this reaction (click here to see animation of reaction).