MUSCLE PHYSIOLOGY

Detailed explanation-1: -Phosphofructokinase-1 (PFK-1) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from ATP to fructose-6-phosphate (F6P) to yield ADP and fructose-1, 6-bisphosphate (FBP). See Glycolysis Enzymes. Mg2+ is also important in this reaction (click here to see animation of reaction).

Detailed explanation-2: -Phosphofructokinase-1 (EC:2.7. 1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to fructose 1, 6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme of glycolysis.

Detailed explanation-3: -Conversely phosphofructokinase is activated by ADP and AMP which serve to indicate that more energy is required. The changes in intracellular ATP and AMP concentrations that occur in anoxia are sufficient to account for the activation of phosphofructokinase and increased rate of glycolysis observed in such conditions.