MUSCLE PHYSIOLOGY

Detailed explanation-1: -Step 10: The final step in glycolysis is the enzymatic conversion of phosphoenolpyruvate to pyruvate by pyruvate kinase. Substrate level phosphorylation occurs in this irreversible step to generate 2 molecules of ATP.

Detailed explanation-2: -During glycolysis, the final step is the conversion of phosphoenolpyruvate to pyruvate with the help of an enzyme.

Detailed explanation-3: -Reaction 9: formation of phosphoenolpyruvate In the ninth step of the glycolytic pathway, 2-phosphoglycerate is dehydrated to form phosphoenolpyruvate, an enol, in a reversible reaction catalyzed by enolase. The reaction requires Mg2+ that stabilizes the enolic intermediate that is formed during the process.

Detailed explanation-4: -Because the substrate for pyruvate kinase is a simple phospho-sugar, and the product is an ATP, pyruvate kinase is a possible foundation enzyme for the evolution of the glycolysis cycle, and may be one of the most ancient enzymes in all earth-based life.