GENETICS AND EVOLUTION
MOLECULAR BASIS OF INHERITANCE
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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14C-labeled CO2
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3H-labeled water
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32P-labeled phosphate
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35S-labeled sulfate
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Detailed explanation-1: -In their experiment, Hershey and Chase used radioactive phosphorous (32P) to specifically label the DNA of the T2 bacteriophage.
Detailed explanation-2: -Since phosphorus is contained in DNA but not amino acids, radioactive phosphorus-32 was used to label the DNA contained in the T2 phage. Radioactive sulfur-35 was used to label the protein sections of the T2 phage, because sulfur is contained in protein but not DNA.
Detailed explanation-3: -(i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell. protein.
Detailed explanation-4: -They labeled the bacteriophage with radioactive isotopes to see where it goes when a virus attacks. They used phosphorus-32 and sulfur-35 because proteins contain no phosphorus (only found in protein) and DNA contains no sulfur (only found in DNA.)