NURSING ANM AND GNM

Detailed explanation-1: -Denaturation of the enzymes generally refers to conformational changes in protein structure & active site of the enzyme and also results in the inactivity of enzymes. Factors that can denature an enzyme are: Enzymes usually require an optimum temperature and pH for their action.

Detailed explanation-2: -Enzymes work best within a certain pH range, and, as with temperature, extreme pH values (acidic or basic) can make enzymes denature.

Detailed explanation-3: -The altering of a protein’s molecular structure is known as denaturation. During denaturation, many of the weak connections and interactions (such as hydrogen bonds) inside a protein molecule that is responsible for the highly ordered structure of the protein in its natural (native) state, are broken.

Detailed explanation-4: -Because enzymes have evolved to function within optimal temperature and pH ranges, once temperature increases and pH changes beyond a certain point, the enzyme becomes denatured. A denatured enzyme refers to an enzyme that has lost its normal three-dimensional, or tertiary, structure.

Detailed explanation-5: -Changes in pH can cause an enzyme to denature (lose their precise shape) and the substrate won’t fit into the active site. Once an enzyme has denatured, it will not function again. The pH at which enzyme activity is highest is known as the optimum pH. Every enzyme will have its own optimum pH.