NURSING ANM AND GNM

Detailed explanation-1: -Enzyme denaturation occurs when an enzyme loses its native conformation, or three-dimensional structure, rendering it unable to bind to substrate and catalyze product formation. The two main causes for enzyme denaturation are deviations from optimal temperature and pH.

Detailed explanation-2: -Enzyme structures unfold (denature) when heated or exposed to chemical denaturants and this disruption to the structure typically causes a loss of activity. Protein folding is key to whether a globular protein or a membrane protein can do its job correctly.

Detailed explanation-3: -Excess heat irreversibly changes the secondary and tertiary structure of the enzyme. This means that its specific shape will be changed and that the amino acids normally located close to each other in the functional active site will no longer be in such a position. Therefore the active site will be non-functional.

Detailed explanation-4: -A denatured enzyme is incapable of providing its necessary function as it cannot actually perform its catalytic function. Denatured enzymes are unable to perform their functions as their ligands (keys) cannot bind to their active sites (locks), and thus no catalysis can actually be performed.