NURSING ANM AND GNM

NURSING EXAM QUESTIONS

BIOCHEMISTRY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Rochelle’s salt is used in which of the following solutions?
A
Fehling’s
B
Benedict’s
C
Seliwanoff’s
D
Molisch’s
Explanation: 

Detailed explanation-1: -Fehling B is Rochelle salt. It is prepared by dissolving potassium tartrate in an aqueous solution of sodium hydroxide. It is also known as potassium sodium tartrate solution. It is a colourless solution and acts as a chelating agent.

Detailed explanation-2: -The Rochelle salt serves as a chelating agent in the solution. Both solution A and B are prepared separately. They are usually kept or stored in a rubber stoppered bottle. So, FehlinQg’s solution is prepared usually when there is a requirement for the solution.

Detailed explanation-3: -Fehling’s A is a solution containing copper(II) sulphate, which is blue. Fehling’s B is a clear liquid consisting of potassium sodium tartrate (Rochelle salt) and a strong alkali, usually sodium hydroxide.

Detailed explanation-4: -The tartrate ions prevent the formation of insoluble Cu(OH)2 from the reaction of copper sulfate and sodium hydroxide present in the solution by forming a bistartarocuprate (II) complex. This complex releases cupric ions slowly for reduction, thus preventing the formation of black cupric oxide.

Detailed explanation-5: -It is used in the electroplating process. It is used in cigarette paper. It is used to break up emulsions. It is one of the ingredients in Biuret reagent to measure the concentration of protein.

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