NURSING ANM AND GNM

Detailed explanation-1: -Imino acids, e.g. proline and hydroxyproline, react with ninhydrin to give a yellow color. At higher temperatures (" 100°C), the yellow compound (X) is trans-formed to the purple-red compound (XI) 1°.

Detailed explanation-2: -Ninhydrin Test Result Interpretation For hydroxyproline and proline, a yellow colour is obtained. For asparagine, brown colour is obtained. If no colour change is observed, the analyte does not contain amino acids, amines, or ammonia.

Detailed explanation-3: -Xanthoproteic Test In this reaction, the aromatic rings become nitrated. When nitric acid is added to a sample and the mixture is heated, a yellow solution will result if the sample contains tyrosine or tryptophan.

Detailed explanation-4: -Additionally, proline is the only amino acid that does not form a red-purple colour when developed by spraying with ninhydrin for uses in chromatography. Proline, instead, produces an orange-yellow colour.