NURSING ANM AND GNM

Detailed explanation-1: -Phosphofructokinase-1 (PFK-1) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from ATP to fructose-6-phosphate (F6P) to yield ADP and fructose-1, 6-bisphosphate (FBP). See Glycolysis Enzymes. Mg2+ is also important in this reaction (click here to see animation of reaction).

Detailed explanation-2: -The enzyme hexokinase phosphorylates or adds a phosphate group to glucose in a cell’s cytoplasm. In the process, a phosphate group from ATP is transferred to glucose producing glucose 6-phosphate or G6P. One molecule of ATP is consumed during this phase.

Detailed explanation-3: -The third step is the phosphorylation of fructose-6-phosphate, catalyzed by the enzyme phosphofructokinase.

Detailed explanation-4: -Phosphofructokinase (PFK) utilizes ATP to phosphorylate fructose-6-phosphate to fructose-1, 6-bisphosphate. As a regulatory enzyme of glycolysis, PFK is negatively inhibited by ATP and citrate and positively regulated by ADP.

Detailed explanation-5: -Phosphofructokinase-1 (EC:2.7. 1.11, PFK-1) catalyzes the phosphorylation of fructose 6-phosphate to fructose 1, 6-bisphosphate using adenosine triphosphate and is a key regulatory enzyme of glycolysis. Mammalian PFK-1 isozymes are composed of three kinds of subunits (PFK-M, -L, and-P), with different properties.