NURSING ANM AND GNM

Detailed explanation-1: -Fehling test is one of the most common tests used for the estimation or identification of reducing sugar and non-reducing sugars. This test by the German chemist H.C. Von Fehling is often used to distinguish between functional groups of ketones and carbohydrates that are water-soluble.

Detailed explanation-2: -These tests use specific reagents known as Benedict’s solution and Fehling’s solution respectively. The main difference between Benedict’s solution and Fehling’s solution is that Benedict’s solution contains copper(II) citrate whereas Fehling’s solution contains copper(II) tartrate.

Detailed explanation-3: -The presence of reducing sugars like glucose can be detected by testing with Fehling’s solution. Glucose is an aldose hexose sugar with a free aldehyde group. It reduces the cupric Cu++ ions in the Fehling’s solution to cuprous Cu+ ions and the solution turns from blue to reddish brown.

Detailed explanation-4: –Benedict’s reagent is a single solution.-Advantages: 1-The alkali is weaker and easier to handle. 2-More sensitive than Fehling’s as it is not readily reduced by urates (as in urine) as Fehling’s reagent.