NURSING EXAM QUESTIONS
BIOCHEMISTRY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Conc Sulfuric acid
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conc nitric acid
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5N Hydrochloric acid
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Conc hydrochloric acid
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Detailed explanation-1: -The reagents consist of resorcinol and concentrated hydrochloric acid: The acid hydrolysis of polysaccharide and oligosaccharide ketoses yields simpler sugars followed by furfural.
Detailed explanation-2: -This test utilizes hydrochloric acid as a dehydrating agent, and the agent resorcinol to condense. When treated with HCL ketoses are dehydrated more quickly, giving a the furfural variant (5-hydroxymethyl fufural) that is then condensed by the chemical resorcinol, resulting in a cherry-red color compound.
Detailed explanation-3: -Seliwanoff’s test is used to distinguish aldoses from ketoses. On treatment with conc. Acid, ketoses are dehydrated more rapidly to give furfural derivatives and on condensation with resorcinol give cherry red complex. The test will be answered by fructose, sucrose and other keto containing carbohydrates.