NURSING ANM AND GNM

Detailed explanation-1: -Methylene Blue is a cationic stain. In simple terms, it is a blue dye that is charged positively and binds the negatively charged components of the cells such as the nucleus, DNA or RNA present in the cytoplasm.

Detailed explanation-2: -It is a cationic dye that stains cells blue because the positively charged dye is attracted to negatively charged particles such as polyphosphates, DNAs, and RNAs. Specimens collected from patients by swabbing are smeared onto microscope slides and the methylene blue solution is dropped on the slide.

Detailed explanation-3: -Notes: Methylene Blue is a cationic stain (positively charged blue dye); and binds to negatively charged parts of the cells, such as nucleus (DNA) and RNA in the cytoplasm (with lower affinity).

Detailed explanation-4: -Methylene blue is a simple and direct stain used for determining bacterial morphology (shape and arrangement). It is a cationic dye (positive charge) which stains the cell a blue color. The presence of negatively charged molecules in the cell (like DNA & RNA) causes the cell to stain blue.

Detailed explanation-5: -Chromophores: Groups with conjugated double bonds that give the dye its color. Direct, cationic, basic or positive dyes: contain positively charged groups. Examples include methylene blue, basic fuchsin, and crystal violet.