NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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put the loop in the tube to get bacteria out
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pass the tube opening through a flame
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open the lid of the petri dish
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pass the loop through a flame
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Detailed explanation-1: -Remove the cap from the tube of the melted agar, and pass the rim of the open tube through the flame of the Bunsen burner. Open the lid of the Petri dish containing your sample and pour the agar in carefully (Panel B of Figure 4). Close the lid then mix the sample with the agar by gently swirling the plate.
Detailed explanation-2: -Never lay the cap down or it may become contaminated. very briefly flame the lip of the culture tube. This creates a convection current which forces air out of the tube and prevents airborne contaminants from entering the tube.
Detailed explanation-3: -Sterilize the inoculation loop after use. If transferring bacteria onto a sterile agar plate, open the lid of a fresh Petri plate with uninoculated agar. Streak the inoculation loop with the bacterial culture back-and-forth across one sector of the agar.
Detailed explanation-4: -Once caps and lids are removed, the lights turn to yellow to indicate an aseptic transfer has not occurred and the medium remains sterile. However, sterility is in jeopardy because the tubes are open and exposed to contamination from the air.