NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Heat the inoculating loop or needle with the bunsen burner flame until it becomes cool to the touch.
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Heat the inoculating loop or needle with the bunsen burner flame until it becomes purple hot.
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Heat the inoculating loop or needle with the bunsen burner flame until it becomes red hot.
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None of the above
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Detailed explanation-1: -After using an inoculating loop or needle to transfer a culture from one medium to the next, what stop should be taken next to avoid contamination. Heat the inoculating loop or needle with the bunsen burner flame until it becomes red hot. When should lab bench tops be cleaned with disinfectant solution?
Detailed explanation-2: -Allow the loop to cool a few seconds before contacting the inoculum to avoid killing the microorganisms.
Detailed explanation-3: -Flame the loop or needle after each inoculation and before a new inoculation is done. This kills any leftover organism that is still on the instrument. Loops are used to transfer from liquid media to liquid media or petri plates. Needles are used to transfer from solid media to other solid media or petri plates.
Detailed explanation-4: -Sterilise the inoculating loop, by heating it in the Bunsen burner flame. Leave it to cool. Alternatively, sterilise it by placing it in pure alcohol for a few seconds. Reason-kills any unwanted bacteria that are present on the loop.