NURSING ANM AND GNM

Detailed explanation-1: -However, extreme high temperatures can cause an enzyme to lose its shape (denature) and stop working. pH: Each enzyme has an optimum pH range. Changing the pH outside of this range will slow enzyme activity. Extreme pH values can cause enzymes to denature.

Detailed explanation-2: -Changes in pH can cause an enzyme to denature (lose their precise shape) and the substrate won’t fit into the active site. Once an enzyme has denatured, it will not function again. The pH at which enzyme activity is highest is known as the optimum pH. Every enzyme will have its own optimum pH.

Detailed explanation-3: -Chemical denaturation of the enzyme was performed with increasing concentrations of the denaturant at pH 4.0.

Detailed explanation-4: -High heat destroys enzymes. Enzymes are protein molecules that get denatured at high temperatures. High heat breaks hydrogen and ionic bonds leading to disruption in enzyme shape. The enzyme loses its activity and can no longer bind to the substrate.

Detailed explanation-5: -As an enzyme is heated beyond its optimum temperature, the hydrogen bonds holding the protein together vibrate and, with increasing temperature, will break. When an enzyme is in a non-optimum pH, the differing proportion of hydrogen ions (which cause changing pH)) will affect those bonds which contain a charge.