NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Intensity of colour is not very dark
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Polychromatic light is used
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Monochromatic light is used
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Both a and b
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Detailed explanation-1: -Polychromatic radiation always gives a negative deviation from Beer’s law, but the effect is smaller if the value of essentially is constant over the wavelength range passed by the wavelength selector.
Detailed explanation-2: -Deviation from Beer’s Law in ultraviolet and visible solution absorptiometry is known to occur due to many factors. Chemical causes include variation in pH, associ-ation or dissociation, solvent-solute interaction, and pho-tochemical reactions.
Detailed explanation-3: -Instrumental Limitations to Beer’s Law For higher concentrations of analyte, less light passes through the sample and PT and Pstray become similar in magnitude. This results is an absorbance that is smaller than expected, and a negative deviation from Beer’s law.