NURSING ANM AND GNM

Detailed explanation-1: -Often the first test performed, gram staining involves the use of crystal violet or methylene blue as the primary color. The term for organisms that retain the primary color and appear purple-brown under a microscope is Gram-positive organisms.

Detailed explanation-2: -The Gram positive cell looses some of its large chunky peptidoglycan cell wall but keeps enough of it to retain the purple colour. The Gram negative cell has its outer membrane and small peptidoglycan layer completely stripped away, leaving it colourless.

Detailed explanation-3: -Gram-positive bacteria characteristically stain purple following the Gram reaction, owing to their thick cell walls; this is in contrast to Gram-negative bacteria, which stain pink or red, owing to their thin cell walls.