NURSING ANM AND GNM

Detailed explanation-1: -Often the first test performed, gram staining involves the use of crystal violet or methylene blue as the primary color. The term for organisms that retain the primary color and appear purple-brown under a microscope is Gram-positive organisms.

Detailed explanation-2: -General description. Gram′s crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity.

Detailed explanation-3: -Gram-positive bacteria have cell walls that contain thick layers of peptidoglycan, a substance that forms the cell walls of many bacteria. The peptidoglycan forms about 90% of the cell wall in gram-positive bacteria. This causes them to appear blue to purple under a Gram stain.

Detailed explanation-4: -Crystal violet has an antibacterial action against Escherichia coli, Staphylococcus aureus, Streptococcus faecalis and Bacillus subtilis. The effect of the dye, measured as minimum inhibitory concentration or retardation of growth, increases as the pH rises from 6 to 8.