NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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30 seconds
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45 seconds
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60 seconds
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it doesn’t matter as long as the specimen is covered
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Detailed explanation-1: -Cover the smear with methylene blue and allow the dye to remain in the smear for approximately one minute (Staining time is not critical here; somewhere between 30 seconds to 2 minutes should give you an acceptable stain, the longer you leave the dye in it, the darker will be the stain).
Detailed explanation-2: -Crystal violet stain is added over the fixed culture. After 10 to 60 seconds, the stain is poured off, and the excess stain is rinsed with water. The goal is to wash off the stain without losing the fixed culture. Iodine solution is used to cover the smear for 10 to 60 seconds.
Detailed explanation-3: -The gram stain utilizes crystal violet as the primary stain. This basic dye is positively charged and, therefore, adheres to the cell membranes of both gram negative and positive cells. After applying crystal violet and waiting 60 seconds the excess stain is rinsed off with water.
Detailed explanation-4: -Stain by covering the smear completely with methylene blue. Allow the stain to act 1-2 minutes. Tilt the slide to allow the stain to drain off. More items •18-May-2021