NURSING ANM AND GNM

NURSING EXAM QUESTIONS

MICROBIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
How many drops of methylene blue is used in the Simeon staining?
A
1
B
2
C
3
D
None of the above
Explanation: 

Detailed explanation-1: -Methylene blue: Prepare a saturated solution of methylene blue by adding 1.5 g powdered methy-lene blue to 100 mL 95% ethyl alcohol. Slowly add the alcohol to dissolve the powder. Add 30 mL saturated alcoholic solution of methylene blue to 100.0 mL distilled water and 0.1 mL 10% potassium hydroxide.

Detailed explanation-2: -Methylene blue staining is useful in determining cell mortality. If methylene blue stain is applied to a sample, a healthy cell with turn the stain colorless. This is due to the cell’s enzymes, which reduce the methylene blue, causing it to lose its color.

Detailed explanation-3: -The methylene blue test is a test to determine the type or to treat methemoglobinemia, a blood disorder.

Detailed explanation-4: -Methylene Blue, 1%, 30 ml. Methylene blue stain is a solution used to highlight certain cell parts of animal, bacteria, and blood tissue specimens. This stain is a 1% aqueous solution, and it’s especially useful for microscope slide preparation.

There is 1 question to complete.