NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In gel electrophoresis, what separates the DNA fragments?
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They are separated by an electrical field
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They are separated by magnetic fields
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They are separated by binding to the gel
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They are separated by the electrical charge of the gel
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Explanation:
Detailed explanation-1: -To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.
Detailed explanation-2: -The molecules separate due to their characteristic charge through the sieve. Thus, within the pool of molecules, size separation is achieved across the gel.
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