NURSING ANM AND GNM

Detailed explanation-1: -Column chromatography separations involve stationary phase being held in a narrow tube (column). The mobile phase or eluent, along with the mixture of components (solutes) is forced through the tube having stationary phase, under pressure.

Detailed explanation-2: -In column chromatography the stationary phase is contained in a tube called the column.

Detailed explanation-3: -In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column. The mobile phase or eluent is a solvent or a mixture of solvents along with substances to be separated and used to move the compounds through the column.

Detailed explanation-4: -Stationary phase in chromatography, is a solid phase or a liquid phase coated on the surface of a solid phase. Mobile phase flowing over the stationary phase is a gaseous or liquid phase. If mobile phase is liquid it is termed as liquid chromatography (LC), and if it is gas then it is called gas chromatography (GC).