NURSING ANM AND GNM

Detailed explanation-1: -The antibacterial action of lysozyme is particularly efficient against Gram-positive bacteria because of its ability to hydrolyze the -1, 4-glycosidic bond present in the polysaccharide layer of these bacteria cell walls.

Detailed explanation-2: -Lysozyme degrades peptidoglycan in the bacterial cell wall leading to rapid killing of Gram-positive organisms; however, this mechanism cannot account for the protective effect of lysozyme against Gram-negative bacteria.

Detailed explanation-3: -Gram-positive bacteria are more susceptible to the action of lysozyme because their cell wall contains up to 90% peptidoglycan, whereas Gram-negative bacteria are more resistant because of the smaller amount of peptidoglycan in their cell wall.

Detailed explanation-4: -Labiase. Labiase from Streptomyces fulvissimus is an enzyme preparation useful for the lysis of many Gram-positive bacteria such as Lactobacillus, Aerococcus and Streptococcus. Labiase contains -N-acetyl-D-glucosaminidase and lysozyme activity.

Detailed explanation-5: -Gram-positive bacteria have a peptidoglycan layer on the outside of the cell wall. Gram-negative bacteria have peptidoglycan between membranes. Penicillin works best on gram-positive bacteria by inhibiting peptidoglycan production, making the cells leaky and fragile.