NURSING ANM AND GNM

Detailed explanation-1: -Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.

Detailed explanation-2: -The partition chromatography principle states that a solute will distribute itself between two immiscible liquids according to its distribution coefficient. The higher the distribution coefficient, the more the solute will partition into the more concentrated liquid.