NURSING ANM AND GNM

NURSING EXAM QUESTIONS

MICROBIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Three cells with a generation time of 30 minutes are inoculated into a culture medium. How many cells (Nt) are there after 5 hours? Use the formula:Nt = N0 x 2n
A
7290
B
1024
C
3072
D
72.9 million
Explanation: 

Detailed explanation-1: -Answer and Explanation: Every 30 minutes, each individual cell will divide into two like-cells; therefore, through 5 hours, 5×2=10 5 × 2 = 10 cell divisions would take place in this case.

Detailed explanation-2: -Generation time (G) is defined as the time (t) per generation (n = number of generations). Hence, G=t/n is the equation from which calculations of generation time (below) derive.

Detailed explanation-3: -So, to get from 100 cells to 800 cells, 100 doubles to 200, which doubles to 400, which doubles to 800. Therefore, 3 generations have passed by in 6 hours. Note that all bacterial generations have the same length, since these do not depend on the number of bacteria. So, the generation time is (6/3) = 2 hours.

Detailed explanation-4: -Generation time is the time it takes for a population of bacteria to double in number. For many common bacteria, the generation time is quite short, 20-60 minutes under optimum conditions. For most common pathogens in the body, the generation time is probably closer to 5-10 hours.

There is 1 question to complete.