NURSING ANM AND GNM

Detailed explanation-1: -Fixation. The fixation procedure is the same regardless of smear source, plate or broth. There are two methods of adhering your bacteria to the slide, heat fixation or methanol fixation. Heat fixing is only used with BSL1 organisms.

Detailed explanation-2: -This technique is used to stain a slide such as a fecal smear to observe the bacterial microflora present based on their gram stain reaction. “Heat-fix” the slide with the specimen by passing it over a heat source, such as a flame, several times using a clothes pin or forceps.

Detailed explanation-3: -Heat fixing kills the bacteria in the smear, firmly adheres the smear to the slide, and allows the sample to more readily take up stains.

Detailed explanation-4: -Bacterial smears are “heat fixed” on the microscope slide before staining to affix the cells to the slide so that they do not wash off during the staining procedure. This step is called fixation and is usually done with the flame of a Bunsen burner.

Detailed explanation-5: -Crystal violet (the primary stain), enters the peptidoglycan of all bacteria giving them a purple color.