NURSING ANM AND GNM

Detailed explanation-1: -For the enhancement of SM production, suspension culture and elicitation are mainly used, but hairy root culture and other organ cultures are proved to satisfy the demand of secondary metabolites.

Detailed explanation-2: -The 17–25∘C range of temperature is judged as the best for the growth of cells as well as accumulation of secondary metabolites.

Detailed explanation-3: -Elevated temperatures increase leaf senescence and root secondary metabolite concentrations in the herb Panax quinquefolius.

Detailed explanation-4: -Strategy to increase production of primary (A) and secondary (B) metabolites. An increase in enzyme production is often achieved by overexpressing the genes encoding the enzymes with the help of promoters of constitutively expressed genes or inducible genes in the presence of specific inducer molecules (e.g., IPTG).