NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Vomiting matters of a patient suspected of having cholera weredelivered to the bacteriological laboratory. The material was used forpreparing a “hanging drop” specimen. What type of microscopy will beapplied for identification of the causative agent by itsmobility?
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Electron microscopy
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Phase-contrast microscopy
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Fluorescence microscopy
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Immersion microscopy
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Explanation:
Detailed explanation-1: -Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet.
Detailed explanation-2: -Optical microscopy is an ideal noninvasive method for studying motility of individual microorganisms. Due to the small physical size of bacteria, imaging them requires high magnification and large numerical aperture objectives.
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