NURSING ANM AND GNM

Detailed explanation-1: -A Gram stain is colored purple. When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative.

Detailed explanation-2: -Gram-positive organisms are either purple or blue in color, while gram-negative organisms are either pink or red in color. Bacilli are rod-shaped, while cocci are spherical.

Detailed explanation-3: -Gram-negative bacteria have cell walls with thin layers of peptidoglycan (10% of the cell wall) and high lipid (fatty acid) content. This causes them to appear red to pink under a Gram stain.

Detailed explanation-4: -Many experts agree that the bacteria that causes these pink stains is most likely Serratia marcescens, a bacteria which is found naturally in soil, food, and in animals. Serratia, which produce a characteristic red pigment, thrive on moisture, dust, and phosphates and need almost nothing to survive.

Detailed explanation-5: -The staining method uses crystal violet dye, which is retained by the thick peptidoglycan cell wall found in gram-positive organisms. This reaction gives gram-positive organisms a blue color when viewed under a microscope.