NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To detect PCR products as they are produced.
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To quantify PCR products
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To detect a viral load
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To amplify RNA viruses
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Detailed explanation-1: -RT–PCR is a variation of PCR, or polymerase chain reaction. The two techniques use the same process except that RT–PCR has an added step of reverse transcription of RNA to DNA, or RT, to allow for amplification.
Detailed explanation-2: -NAATs such as RT-PCR for SARS-CoV-2 are designed to detect viral RNA. A positive result is highly specific for the presence of viral nucleic acid; however, it does not differentiate between viable and nonviable virus.
Detailed explanation-3: -This sample contains both the person’s and the virus’s RNAs. The reverse transcription-polymerase chain reaction test is also known as the RT-PCR test. It is a gold-standard test for diagnosing viral diseases like Ebola and SARS-CoV-2 virus infection. The RT-PCR is a sensitive test.