NURSING EXAM QUESTIONS
MICROBIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Substance for separation
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Substance for impurity
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A and B
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None of the above
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Detailed explanation-1: -Analyte – the substance to be separated during chromatography. It is also normally what is needed from the mixture. Analytical chromatography – the use of chromatography to determine the existence and possibly also the concentration of analyte(s) in a sample.
Detailed explanation-2: -In gas chromatography, the components of a sample are dissolved in a solvent and vaporized in order to separate the analytes by distributing the sample between two phases: a stationary phase and a mobile phase.
Detailed explanation-3: -Analytes (substances being analyzed) are separated from each other based on a differential affinity to a solvent. The solvent dissolves and carries the analytes along the matrix of the stationary phase. Since the solvent moves through a wicking action, it is called the mobile phase.