NURSING ANM AND GNM

Detailed explanation-1: -MALDI – Principle and Methodology. The sample for analysis by MALDI MS is prepared by mixing or coating with solution of an energy-absorbent, organic compound called matrix. When the matrix crystallizes on drying, the sample entrapped within the matrix also co-crystallizes.

Detailed explanation-2: -Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.

Detailed explanation-3: -MALDI-TOF MS determines the unique proteomic fingerprint of an organism, and matches characteristic patterns with an extensive reference library to determine the organism´s identity. Continuous expansion of the reference library ensures that a broad range of microorganisms can be identified easily.

Detailed explanation-4: -Laboratories must perform internal QC before using MALDI-TOF MS to identify microorganisms. Internal QC consists of an automatic instrument calibration using a manufacturer-specified calibration standard. Depending on the system, calibrators include a manufactured extract of Escherichia coli (E. coli) or a specific E.