NURSING ANM AND GNM

NURSING EXAM QUESTIONS

MICROBIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
When is electrophoresis not used?
A
Separation of proteins
B
Separation of amino acids
C
Separation of lipids
D
Separation of nucleic acid
Explanation: 

Detailed explanation-1: -Lipid as its can not be move in electrophoresis because they are not charged molecules. But you can follow a procedure of ultacentrifugation of serum to separate lipoproteins then you can quantify them by gel electrophoresis.

Detailed explanation-2: -Consequently, unlike nucleic acids, proteins cannot be effectively separated by electrophoresis on agarose gels. To separate proteins by electrophoresis, one must make several modifications.

Detailed explanation-3: -If it doesn’t have in charge then it will not move towards the positive or negative electrons at such. So if we talk about carbohydrates or carbohydrates are usually negatively charged. So they have negative charge on them. Whereas if we talk about fats they do not have any charge.

Detailed explanation-4: -Polyacrylamide gel electrophoresis was found to be a rapid, reliable, and satisfactory procedure for plasma lipo-protein phenotyping. In view of the association of lipid disorders and coronary heart disease, lipid and lipoprotein assays have become an increasingly large part of the routine laboratory workload.

There is 1 question to complete.