NURSING ANM AND GNM

Detailed explanation-1: -The final step in gram staining is to use basic fuchsin stain to give decolorized gram-negative bacteria pink color for easier identification. It is also known as counterstain. Some laboratories use safranin as a counterstain; however, basic fuchsin stains gram-negative organisms more intensely than safranin.

Detailed explanation-2: -A counterstain, such as the weakly water soluble safranin, is added to the sample, staining it red. Since the safranin is lighter than crystal violet, it does not disrupt the purple coloration in Gram positive cells.

Detailed explanation-3: -The safranin is employed as a counter-stain in endospore staining and Gram’s staining. It is mostly utilized for the identification of cartilage, mucin, and mast cell granules. The safranin stain works by binding to acidic proteoglycans in cartilage tissues with a high affinity forming a reddish orange complex.

Detailed explanation-4: -The first step in gram staining is the use of crystal violet dye for the slide’s initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet-iodine complex to prevent easy removal of dye.

Detailed explanation-5: -Applying a primary stain (crystal violet). Adding a mordant (Gram’s iodine). Rapid decolorization with ethanol, acetone or a mixture of both. Counterstaining with safranin. 16-Mar-2022