NURSING EXAM QUESTIONS
MICROBIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Filter paper
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Nitrocellulose membrane
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Cellulose acetate
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None of the above
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Detailed explanation-1: -Polyvinylidene difluoride (PVDF) membrane is ideal for western blotting applications as well as for amino acid analysis and protein sequencing of small amounts of proteins (as little as 10 pmoles). In addition, PVDF membranes can be used, stripped and reprobed without a loss of sensitivity or increased background.
Detailed explanation-2: -Nitrocellulose membranes, one of the most important and oldest cellulose derivatives, are commonly used for nucleic acid and protein detection in research and diagnostic applications.
Detailed explanation-3: -While nitrocellulose is brittle and fragile, PVDF is more durable and has higher chemical resistance making it ideal for reprobing and sequencing applications. Nitrocellulose can prove to be difficult to strip and reprobe without losing signal.