NURSING ANM AND GNM

Detailed explanation-1: -In bacteria, the cell wall forms a rigid structure of uniform thickness around the cell and is responsible for the characteristic shape of the cell (rod, coccus, or spiral). Inside the cell wall (or rigid peptidoglycan layer) is the plasma (cytoplasmic) membrane; this is usually closely apposed to the wall layer.

Detailed explanation-2: -Because of the peptidoglycan layer. The thickened peptidoglycan layer in Gram positive cells allows them to retain the stain (hence remaining ‘stain positive’ or ‘Gram positive) where as the thin layer seen in Gram negative cells cannot prevent the stain from leeching out (hence stain and Gram negative).

Detailed explanation-3: -Introduction. Peptidoglycan (murein) is an essential and specific component of the bacterial cell wall found on the outside of the cytoplasmic membrane of almost all bacteria (Rogers et al., 1980; Park, 1996; Nanninga, 1998; Mengin-Lecreulx & Lemaitre, 2005).

Detailed explanation-4: -The crystal violet dye adheres to the thick peptidoglycan layer of the cell wall present in gram-positive bacteria, staining them violet or purple when visualized under a light microscope.

Detailed explanation-5: -Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.