NURSING ANM AND GNM

Detailed explanation-1: -Annealing stage This enables the primers to attach to a specific location on the single-stranded template DNA by way of hydrogen bonding (the exact temperature depends on the melting temperature of the primers you are using).

Detailed explanation-2: -Annealing, or hybridization, is the spontaneous pairing of complementary DNA or RNA sequences by hydrogen bonding to create a double stranded molecule. The reaction mixture is cooled to a temperature of 50-60°C for 20-40 seconds.

Detailed explanation-3: -PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.