NURSING ANM AND GNM

Detailed explanation-1: -Petri dishes need to be incubated upside-down to lessen contamination risks from airborne particles landing on them and to prevent the accumulation of water condensation that could disturb or compromise a culture.

Detailed explanation-2: -Agar plates must be incubated upside down to prevent the condensation that occurs on the lid to drip onto the culture. Condensation will interfere with colonial growth and may introduce a contaminant.

Detailed explanation-3: -Store plates upside down in a refrigerator or cold room. If they are stored in a room, check the plates after a few hours for condensation in the lid. If you have the plates upside down and there is condensation in the lid, there must be some heat source above that is driving water out of the agar and into the lid.

Detailed explanation-4: -Why do you label plates on the bottom, not on the lid? After the culture medium is set, and streaked with the required microbe/stock, the lid is put on and the petri dish is incubated upside down to minimize contamination. So, it is easier to read the label on the bottom.

Detailed explanation-5: -Placement of polymer discs on the top of Petri dishes is a relatively simple method that can be used to buffer the lid from small temperature changes and minimize condensation problems. all temperature changes and minimize condensation problems.